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Aspartame (APM) is widely used as a sugar substitute in the United States. APM is a potent agonist of mammalian sweet taste receptors (TAS1R2 and TAS1R3). Although APM is a non-cariogenic sweetener, some persons experience nocturnal hypoglycemia or ketosis after using APM. The purpose of this study was to determine whether the ingestion of a diet containing APM would alter the endocrine responses to a test meal. We studied 30 lean young adults, aged 18-36 y, on 2 successive days. On each day, participants consumed a low-calorie test meal in the morning after an overnight fast (approximately 12-h) and then consumed the same test meal again with APM or an isocaloric control beverage at midafternoon. On the second day, all participants consumed a test meal with APM after a longer fast of 4 h. All foods were in place at all times. The primary outcome of the study was insulin response to the test meal. The secondary outcome was blood glucose and plasma free fatty acid (FFA) response to the test meal. Results showed that during the fasted condition, ingestion of APM led to a decrease in both blood glucose and plasma FFA levels, and a decrease in insulin and an increase in glucose levels after the test meal. After 4 h of fasting, levels were unchanged. We conclude that APM is not a specific sweetener; it may actually slow the endocrine response to a test meal after only a short fast.From playing the “Tornado” golf course, to fun family events such as the annual Autumn Party (and the